This presentation explores the potential of mesophilic carotenogenic microalgae for beta-carotene production. While *Dunaliella* is a common source of natural beta-carotene, it has drawbacks such as high water loss, difficult biomass harvesting, and the need for salt-rich media. Viable alternatives like *Bracteacoccus aggregatus* and *Coelastrella rubescens*, also green algae, offer advantages such as lower salt requirements and suitability for photobioreactor cultivation, making them promising candidates for sustainable beta-carotene production.